SL3-3 infection leads to various subtypes of T-cell lymphomas in wild-type and NFATc2- or NFATc3-deficient mice. Flow cytometry analysis of cells from (A) spleen of mock-infected Nfatc3^-/- mice, (B) spleen lymphoma from a SL3-3–infected Nfatc3^-/- mouse, (C) thymus lymphoma from an SL3-3–infected wild-type mouse, and (D) spleen lymphoma from an SL3-3–infected Nfatc2^-/- mouse. (A-B) To distinguish between T cells, myeloid cells, and B cells, cells were stained with a mixture of anti-CD3–PE, anti-CD11b–FITC, and anti-CD45/B220–PerCP Abs, respectively. In panels C and D, a mixture of anti-CD3–PE, anti-CD4–FITC, and anti-CD8–PerCP–Cy5.5 antibodies was used to distinguish among T-cell subtypes. Two different populations of T cells in panel D are indicated with rectangles 1 and 2 representing CD3⁺CD4⁺CD8⁺ cells and CD3⁺CD4^-(dim)CD8^+(dim) cells, respectively. Note that parallel analysis of both thymus and spleen tumors derived from the same animal revealed basically identical FACS profiles in most of the cases investigated. Five percent probability contour plots are shown. Grids are positioned according to background fluorescence of isotype controls. Numbers in quadrants indicate the percentage of cells within the limits of the quadrants. For a summary of tumor phenotypes, see Table 2.