Similar patterns of Rel/NF-κB complexes are induced in cDCs and pDCs by CpG DNA. (A) Rel/NF-κB complexes induced in response to CpG. Nuclear extracts isolated from cDCs (lanes 1 and 2) and pDCs (lanes 3 and 4) prior to (lanes 1 and 3) and after CpG1668-ODN–induced activation (2 hours; lanes 2 and 4) were subjected to electrophoretic mobility shift assays (EMSA). (B, C) Antibody shifts of Rel/NF-κB complexes in CpG-activated cDCs and pDCs. Preimmune sera (lane 1) or antibodies specific for NF-κB1 (lane 2), c-Rel (lane 3), RelA (lane 4), and p52NF-κB2 (lane 5) were first incubated with nuclear extracts from wt cDCs and pDCs stimulated for 2 hours with CpG prior to performing EMSA.