Effects of NGF and CGRP_8-37 on B7 expression. A representative finding of 8 independent experiments showing CD80 and CD86 expression in peripheral-blood monocytes freshly taken (t₀: A,G) or after 24 hours of different culture conditions: unstimulated cells (Us: B,H), LPS-treated monocytes (LPS: C,I) with or without the addition of anti-NGF antibody (LPS abNGF: D,J), control IgG (LPS IgG: E,K), CGRP_8-37, the CGRP₁ receptor antagonist (LPS CGRP_8-37: F,L). Negative controls were cells incubated with isotype mouse IgG (darker area). For each population the values of ΔMFI for CD80 and CD86 positivity are included.