Cell cycle analysis of purified mouse c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells after mobilization with combination of CTX and G-CSF. Mice were treated with CTX (200 mg/kg) and, 24 hours later, infused continuously with G-CSF (190 μg/kg/d) for 5 (Exp. IV and V) or 7 days (Exp. VI). c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells were purified and the cells were stained with Hoechst 33342 for analysis of DNA content. The FACS plots from three separate experiments show Hoechst 33342 staining versus forward scatter of c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells isolated from (d and g) BM, (b, e, and h) spleen, and (c, f, and g) MPB. There were not enough KTLS cells isolated from BM during Exp. IV for analysis. The percentage of cells in G0/G1 or S/G2/M is indicated on the left or right side of each FACS plot, respectively.