Cell cycle analysis of human CD34⁺ progenitors from BM and MPB after mobilization with G-CSF or CTX/G-CSF. The CD34⁺ subset of cells was obtained stained with Hoechst 33342 and antibodies specific for Thy-1. The FACS plots of the CD34⁺ gated populations show Hoechst 33342 staining versus Thy-1 expression from cells isolated from (a) BM from an untreated donor, (b) MPB from a donor treated with G-CSF, and (c) MPB from a patient treated with a combination of CTX/GM-CSF. The percentage of CD34⁺ cells is shown in each quadrant of the FACS plot.