ASRA of six dimorphic sites in the factor V gene. Six restriction sites in exons 2, 4, 9, 11, 13, and 16 of the factor V gene were characterized using standard PCR techniques and the enzymes Acc I, Pst I, Ear I, Hph I, HinfI, and Nde I, as indicated (see the Materials and Methods). In each case, the site was scored positive (+) if it was cleaved and negative (−) if it was not. Examples of typical cleavage patterns, eg, +/+, +/−, and −/−, for individual subjects for each dimorphism are given. Numbers alongside the gel photos indicate the size (in basepairs) of the bands.