Fig. 1.
![Fig. 1. Activation of IRE-binding activity of IRP by Epo in K562 cells. K562 cells were treated for 24 hours with 50 μmol/L Fe(NO3 )3 (I), 100 μmol/L desferrioxamine (D), 50 U/mL human recombinant Epo (Epo), or 1.5% DMSO or remained untreated (control [C]). Fifteen micrograms of detergent cell extracts was assayed for IRE-binding activity of IRP by means of gel shift assay in the absence (upper panel) or presence of 2% 2-mercaptoethanol. After fixation, gels were exposed for 6 to 48 hours to x-ray films. The IRE/IRP complex and the nonbound IRE-probe (free probe) are shown in the upper panel, whereas in the lower panel only RNA-protein complexes are pictured. The results of one of seven similar experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.680/4/bl_0026f1.jpeg?Expires=1735843231&Signature=AmerDplGfZzXAKSoPIhLYmaZPf3~KXYgHpuwri--z8MFkB-hugqMwU04mBtLOe1xOxmn~xFDUc3wf1mygSE5f-CEZxTe6erl6E8pe82TUbch0uBC9IWf-o9dg7v~aef0BqtJ2iL73o6ktqKV-WfVtoOm~oh-T2COi-6GCWWar-WLXewk8hpd4u1nGH8zwnYnDi9NoFx5iKW9oob5Qy9pq6bP0G0iB8g70M7gzF3fCxj1-7UVTtLkJSDLsAnPpg8z6dYJmrW8NwX4Xg4WYyyuz4QVBcEK9iXqCaoEyp5xk1lSqB9JjqRLhLbbGc76vqeCPhbKQGuTn2h3tUj2G09-Fw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of IRE-binding activity of IRP by Epo in K562 cells. K562 cells were treated for 24 hours with 50 μmol/L Fe(NO3 )3 (I), 100 μmol/L desferrioxamine (D), 50 U/mL human recombinant Epo (Epo), or 1.5% DMSO or remained untreated (control [C]). Fifteen micrograms of detergent cell extracts was assayed for IRE-binding activity of IRP by means of gel shift assay in the absence (upper panel) or presence of 2% 2-mercaptoethanol. After fixation, gels were exposed for 6 to 48 hours to x-ray films. The IRE/IRP complex and the nonbound IRE-probe (free probe) are shown in the upper panel, whereas in the lower panel only RNA-protein complexes are pictured. The results of one of seven similar experiments are shown.
