Fig. 4.
Induction kinetics of IRPs by Epo in MEL cells. Cells were treated for 24 hours with 50 μmol/L Fe(NO3 )3 (I), with 100 μmol/L desferrioxamine (D), and, for the indicated times, with murine recombinant Epo (0.5 U/mL) or remained untreated (control [C]). Gel retardation assays were performed exactly as described in the legend to Fig 1. The results of one of three representative experiments are shown.