Fig. 5.
Increase of trf-rec mRNA concentration upon Epo-treatment of K562 cells. K562 cells were treated for 24 hours with 50 μmol/L Fe(NO3 )3 (I), 100 μmol/L desferrioxamine (D), or the indicated concentrations of human recombinant Epo (Epo) or remained untreated (control [C]). The total RNA was then extracted and 10 μg was subjected to Northern blotting with a human trf-rec cDNA or a rat GAPDH probe. The results of one of three similar experiments are shown.