Fig. 6.
Modulation of Trf-rec mRNA concentration upon treatment of MEL cells with EPO. MEL cells were treated for 16 hours with 50 μmol/L Fe(NO3 )3 (I), 100 μmol/L desferrioxamine (D), or, for the indicated times, with 0.5 U/mL of murine recombinant Epo (Epo) or remained untreated (control [C]). The total RNA was then extracted and 10 μg was subjected to Northern blotting with a murine trf-rec cDNA or a rat GAPDH probe. The results of one of four similar experiments are shown.