Fig. 7.
![Fig. 7. Scatchard analysis of transferrin binding to K562 cells. Cells were preincubated for 24 hours with 50 U/mL human recombinant Epo (Epo), 100 μmol/L desferrioxamine (Dfo), or no addition (C) and were then incubated for 30 minutes on ice with 125I-transferrin. Nonspecific binding was determined by incubating with a 200-fold excess of unlabeled transferrin. The number of bindings sites per cell after correcting for nonspecific binding were, for control, n = 0.45 × 105 (dissociation constant [kd] = 41 nmol/L); for Epo-treated cells, n = 1.11 × 105 (kd = 43 nmol/L); and for desferrioxamine-treated cells, n = 2.92 × 105 (kd = 43 nmol/L). The results of one of three similar experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.680/4/bl_0026f7.jpeg?Expires=1735842616&Signature=dJfZaFB~wya22YQgaVgJGYsyRdUxFNq0KiAJHrSDSOCEmwIOnHl1APMZyFB1MDYDEGXAhAWIWmthgUUuCGgqIVeLJKJq-a-hlz8kVV6a8oyykAr8iVFw5dcfpJLNRRhs4Y6DfkfLxUyoU5Zfg9pJ56m~IEJhhPU3RaSt16PH4ZDwaBrc4pXaWMoAAGH3B4-xJtPUFkKV82soUPW~fhHGW~7DVtsyZCDgoTajY546XVRuMG56vivHZIj6CC84rWYYJWt9bjx6EkogRWEEhUKfappqSteuY3B-7GWctZyT5qdOr~ZDt3dcRSxaww-8DypiZODEcCqT9irmJe1SxPOSYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Scatchard analysis of transferrin binding to K562 cells. Cells were preincubated for 24 hours with 50 U/mL human recombinant Epo (Epo), 100 μmol/L desferrioxamine (Dfo), or no addition (C) and were then incubated for 30 minutes on ice with 125I-transferrin. Nonspecific binding was determined by incubating with a 200-fold excess of unlabeled transferrin. The number of bindings sites per cell after correcting for nonspecific binding were, for control, n = 0.45 × 105 (dissociation constant [kd] = 41 nmol/L); for Epo-treated cells, n = 1.11 × 105 (kd = 43 nmol/L); and for desferrioxamine-treated cells, n = 2.92 × 105 (kd = 43 nmol/L). The results of one of three similar experiments are shown.
