IRP activity in monocytes of controls, GH and SH patients. Autoradiogram of a representative RNA-bandshift assay. Lysates of monocytes from controls (lanes 1 and 2), patients with SH due to sporadic PTC (lane 3), and unrelated GH patients treated (lanes 4 to 6) and untreated (lanes 7 to 10), were incubated with a 32P-labeled riboprobe spanning the IRE of the ferritin H chain in the absence (top panel) or presence (bottom panel) of 2% 2-mercaptoethanol. RNA-protein complexes were separated on nondenaturing acrylamide gels as described in Materials and Methods. The exposure time of the autoradiogram shown in the top panel was twice longer. IRP activity was higher in untreated GH patients than in controls and SH patients, whereas no significant difference was found between treated GH patients and controls.
