Fig. 3.
Effect of supernatant (sn) from HUVEC/M coincubation on TF activity in HUVECs and M. HUVEC (2 × 105/well) and M (4 × 105/well) were incubated together for 4 hours at 37°C. At the end of incubation the supernatant was collected, sedimented by centrifugation, and added to HUVEC or M obtained from the same donors and cultured in parallel. The cells were then washed and tested for TF activity, as described in Materials and Methods. Results are expressed in percent of TF activity. A 100% value was assigned to the activity expressed by the HUVEC/M coculture. Each column represents the mean of seven experiments ± SEM. *P < .01 when compared with HUVEC + M.
