Fig. 6.
![Fig. 6. Fit of data obtained from in vitro cell assays to a mathematic model describing antibody-mediated receptor dimerization. Determination of KD by BIAcore analysis showed a value of ∼84 nmol/L. Baseline values for proliferation ([3H]thymidine incorporation in the absence of MoAb34) were subtracted. (A) Proliferation of BaF3/EPO-R cells; (B) proliferation of UT-7/EPO cells; (C) differentiation of CD34+ cells. The mean number of colonies obtained in the 2 counts of experiment 2 (Table 2) was used.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.473/4/bl_0016f6.jpeg?Expires=1735842411&Signature=zIZ4WYXfpt7sTli8bKF~-75nj8HQrRIUchX~p70~qQ1vsCpgPiY9dGK9e2WRcM-gmYCVvZcQCMe6lUQaFAo-Vj-b81JiKRu090WNOckK7VuF--3069Zv2i77wWIpaQJZP~zmOgMA74viwNMT4OQaqWCSVlto1MIUlnSbPCAQxaWdVLxdbGeblBZmY-TFFgCioIihUJiVr9NOM7KnxUHHdFH-rtZKmlSfrI8ee~5cLY9y21TmwMbvsv1R5dlvT7iYb8uobEW0Lg8P7p1AEQLr~XIrTIpRrNcf0IkQkrePm79C-PIICWr5x5Ocyc~-jQHnPSOPWgk~acr1W0ucIQ8bhQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fit of data obtained from in vitro cell assays to a mathematic model describing antibody-mediated receptor dimerization. Determination of KD by BIAcore analysis showed a value of ∼84 nmol/L. Baseline values for proliferation ([3H]thymidine incorporation in the absence of MoAb34) were subtracted. (A) Proliferation of BaF3/EPO-R cells; (B) proliferation of UT-7/EPO cells; (C) differentiation of CD34+ cells. The mean number of colonies obtained in the 2 counts of experiment 2 (Table 2) was used.
