Characterization of mRFC-transduced MOLT-3/MTX_10,000 cells. (A) MTX uptake in mRFC-transduced MOLT-3/MTX_10,000 cells (MTXR/mRFC) and control cells transduced with empty vector (MTXR/pLXSN). (B) Cytotoxicity curves of mRFC-transduced MOLT-3/MTX_10,000 cells (MTXR/mRFC) against MTX in medium containing folic acid compared with MOLT-3/MTX_10,000 cells transduced with an empty vector (MTXR/pLXSN). (C) Cytotoxicity curves of mRFC-transduced MOLT-3/MTX_10,000 cells against TMTX in medium containing folinic acid compared with MOLT-3/MTX_10,000 cells transduced with an empty vector. Each point represents the average of two to three separate experiments performed in triplicate.