Phenotypic analysis of B-cell compartments in FL and day 5 bone marrow and spleen from wildtype and W/W mice. (A) Analysis of B lineage subpopulations present in day 16 and day 18 fetal liver (FL) of wildtype and W/W mutant mice. Total cell numbers are indicated on top of each column. B lineage cells were analyzed by two-color flow cytometry for expression of B220 versus IgM (top row) and by four-color flow cytometry for expression of B220 versus CD43 versus BP-1 versus HSA. Data are presented as expression of B220 versus CD43 of the whole population of nucleated cells (middle row) and BP-1 versus HSA gated on B220⁺CD43⁺ cells (bottom row). Numbers given in the individual quadrants indicate percentages. (B) Analysis of B lineage subpopulations present in bone marrow (BM) and spleen (SP) of day 5 wildtype and W/W mutant mice. The data are presented in the same way as for (A).