Analysis of the contribution of transferred wildtype or W/W FL cells to B-cell and myeloid development in the bone marrow of reconstituted RAG-2^−/−. A total of 8 × 10⁵ FL cells from day 13 wildtype or W/W mice were injected intravenously into 4Gy irradiated RAG-2^−/− mice, and reconstituted mice were analyzed 3 weeks posttransfer. Bone marrow cells from Ly5.2⁺RAG-2^−/− mice reconstituted with Ly5.1⁺ wildtype FL cells (A through F ) or with Ly5.1⁺W/W FL cells (G through L) were analyzed by three-color flow cytometry for the expression of Ly5.2, CD43, and B220. Total cell counts are shown on top of (A) and (G). Bone marrow cells are gated, according to forward/side scatter analysis, into nonlymphoid (R1) and lymphoid (R2) gates. Cells in the R1 and R2 gates are further analyzed for the expression of B220 versus CD43 (B, C, H, and I). Percentage of donor-type cells (Ly5.2⁻) are determined among the nonlymphoid (D and J), B220⁺CD43⁺ early B-cell precursor (E and K) and B220⁺CD43⁻ late B-cell precursor (F and L) populations. Numbers given on the gates indicate percentages.