Fig. 2.
![Fig. 2. E64d blocks anti-CD3–induced upregulation of Fas ligand protein. 2B4.11 cells were activated with the indicated concentrations of plastic-coated 2C11 in the presence or absence of 100 μmol/L E64d for 2 hours. [3H]thymidine-labeled L1210 cells or L1210-Fas cells were then added to the cultures. The parentheses in the legend indicate the time of addition of E64d: (2B4.11 + E64d), E64d added at time 0; (E64d + L1210-Fas), E64d added with the L1210-Fas cells, 2 hours after initiation of the culture. After another 6 hours of incubation, the cells were harvested and the percentage of DNA fragmentation determined. Similar results were obtained in three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.550/4/bl_0001f2.jpeg?Expires=1735844032&Signature=TgmAf3fPLJRyTD05o0v9Sip3yGA0OO-LdljCxUc3p0dsHwxk8Rixt2qhMKIcq2fyMRi-TIoKM7UxP~skC9moCcilTNXLSQgSw~tJDuYhRG59XwcN7QN~Pvg6JOA5-8mBHYUz-zN~qgpFpadAJfKpBDGBTwQQitKdRoufyABac8zfi8LBlp2zBCOGwCN5rn9gEXUAgz4yspvDoJtgz8KsV6sGWBLvmFZQRIOEks~MQN3DsNAIFK-fJUrMSK8wkTEGjkdIUyeVFLUHAzvN3z38TdGW4dKkZ7ZmPpd3HVQIjMR8-hv2xUtX8qUEv-Hz8N6kU6A2OKIq7r7S0p2o4zztHQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
E64d blocks anti-CD3–induced upregulation of Fas ligand protein. 2B4.11 cells were activated with the indicated concentrations of plastic-coated 2C11 in the presence or absence of 100 μmol/L E64d for 2 hours. [3H]thymidine-labeled L1210 cells or L1210-Fas cells were then added to the cultures. The parentheses in the legend indicate the time of addition of E64d: (2B4.11 + E64d), E64d added at time 0; (E64d + L1210-Fas), E64d added with the L1210-Fas cells, 2 hours after initiation of the culture. After another 6 hours of incubation, the cells were harvested and the percentage of DNA fragmentation determined. Similar results were obtained in three independent experiments.
