Fig. 4.
Competitive PCR for FasL mRNA in fresh and cultured PBL. mRNA in nonnuclear lysates of fresh or cultured (20 hours) PBL from an HIV-infected donor was reverse-transcribed using random primers and amplified by PCR in the presence of increasing amounts of mutated cDNA (lane 1 through lane 6, the amounts of mutated cDNA are 10−3, 2 × 10−3, 4 × 10−3, 10−2, 2 × 10−2, and 4 × 10−2 amol, respectively). The PCR products were analyzed by agarose gel electrophoresis. The wild type cDNA is seen as a 241-bp band, while the mutated cDNA migrates as a 214-bp band.
