Replication of HIV-1 in transduced, G418-selected LTBMC. (A) LTBMC established from transduced, G418-selected CD34⁺ cells of HIV-1–infected individuals were inoculated with HIV-1_JR-FL . Supernatant samples taken weekly were assayed for the amount of HIV-1 core protein (p24) produced after HIV-1 infection. Cells were transduced with the LN vector (□), the L-RRE-neo vector (▴), the L-TR/TAT-neo vector (▪), and the L-M10 SN vector (○). (B) LTBMC established from transduced, G418-selected CD34⁺ cells were inoculated with an HIV-1 primary isolate, and the same sampling and p24 assay procedures as above were applied. Cells were transduced with the LN vector (□), the L-RRE-neo vector (▴), the L-TR/TAT-neo vector (▪), and the L-M10-SN vector (○).