Frequency of cytokine secreting cells in transgenic mice versus normal littermate controls. ELIspot assays were used to determine the number of cells secreting IFN-γ (A), IL-12 (B), and IL-4 (C) in the bone marrow (BM), thymus (Thy), spleen (Spl), and mesenteric lymph nodes (LN) of transgenic mice (TG) and normal littermate controls (N). Results represent the individual frequencies of cytokine producing cells of 3 mice per group.