Effect of calcium ionophore on the viability on FDC-P1 transfectants expressing or not expressing mcl-1. (A) Photographs of clone 7.5-MAMmcl-1 either left untreated (Control) or exposed to 1.5 μmol/L A23187 in the absence (−Dex) or presence of dexamethasone (+Dex), as assessed by the ethidium bromide/acridine orange method. Arrows indicate cells that have condensed nuclei but have not lost membrane integrity. Photographs were taken at a magnification of 400×.
(B) Clones 7.5-MAMmcl-1 and 8.5-MAMneo were either incubated (+) or not incubated (−) with dexamethasone for 2 hours at an inoculum density of 5 × 105 cells/mL. A23187 (1.5 μmol/L) was added and cell viability was was assayed at the indicated times using the ethidium bromide/acridine orange method. The values shown are the mean ± SD of three independent experiments.
