rhIL-6 stimulates pGL2/PLPR promoter activity in Hep3B cells. Hep3B cells, grown to approximately 90% confluence in 100-mm dishes, were transiently transfected with the indicated plasmid constructs using 40 μg of plasmid DNA and 125 μL lipofectamine reagent. Cells were grown in EMEM containing 10% FCS and cultured in the presence of either media alone or 500 U/ml rhIL-6 for 24 hours in EMEM containing 10% FCS. Protein concentrations of cell lysates were determined and an appropriate volume of lysate was added to 100 μL of luciferase assay reagent (Promega) so that 300 μg of protein were assayed for each experimental condition. Luciferase activity was measured and expressed as counts/300 μg (10 s−1) protein. Error bars represent standard deviation.
