Time dependence of the effect of rhIL-6 injection on hepatic plasminogen mRNA expression in mice. (A) C57BL/6J male mice were injected intraperitoneally with either saline (T = 0) or 4,000 U rhIL-6 and killed at the indicated times. Livers were procured and total RNA was extracted and subjected to Northern blot analysis using the 32P-cDNA probes for murine plasminogen and for murine cyclophilin. The time course points were determined at least twice. Each lane represents RNA isolated from an individual representative animal. Migration of RNA molecular size standards is shown on the left. (B) Fold-stimulation was determined as the ratio of plasminogen/cyclophilin hybridization band intensity determined by laser densitometric scanning of the autoradiogram in (A). The ratio was normalized to one for the untreated condition.
