Fig. 1.
Polyclonal anticortactin antibody detects specifically cortactin proteins in murine fibroblasts. Murine fibroblasts (1 × 106 3T3 cells) were directly lysed in 300 μL of the SDS sample buffer. Ten microliters (lane 1) and 20 μL (lane 2) of the lysate were analyzed by Western blotting using purified polyclonal anticortactin antibody (C001) at the concentration of 1 μg/mL.