American Society of Hematology

Fig. 5.

$Fig. 5. Tyrosine phosphorylation of cortactin in CMK cells stimulated with PMA. (A) CMK cells were treated with PMA for the time periods as indicated, lysed, and subject to immunoprecipitation with the polyclonal antibody 2719 against a cortactin-specific peptide.23 The immunoprecipitates were fractionated with an SDS-PAGE (7.5%) and immunoblotted with an antiphosphotyrosine antibody (anti–p-Tyr). (B) The membrane used above was stripped and reblotted with 4F11 monoclonal antibody. (C) CMK cells stimulated with PMA in parallel were lysed directly with SDS-sample buffer and analyzed for c-Src–related kinases using c-Src-2 antibody.$

Tyrosine phosphorylation of cortactin in CMK cells stimulated with PMA. (A) CMK cells were treated with PMA for the time periods as indicated, lysed, and subject to immunoprecipitation with the polyclonal antibody 2719 against a cortactin-specific peptide.23 The immunoprecipitates were fractionated with an SDS-PAGE (7.5%) and immunoblotted with an antiphosphotyrosine antibody (anti–p-Tyr). (B) The membrane used above was stripped and reblotted with 4F11 monoclonal antibody. (C) CMK cells stimulated with PMA in parallel were lysed directly with SDS-sample buffer and analyzed for c-Src–related kinases using c-Src-2 antibody.

This Feature Is Available To Subscribers Only