Reduction of RARα protein by the RARα antisense construct and compensatory increase in the RARβ and RARγ proteins. Western blot analysis of protein isolated from normal control (N) mice and transgenic (T) mice (line 2) from the indicated organs. Equal amounts of protein were resolved on SDS-PAGE, transferred to nitrocellulose membrane, and probed with specific RARα (A), RARβ (B), and RARγ (C) antisera as indicated. The percentage of RARα reduction in each tissue is indicated in (A) and the levels of increase in RARβ and RARγ proteins are shown in (B and C).