Fig. 9.
CD95-dependent cell-mediated cytotoxicity of Th1 and Th2 clones on Jurkat cells. Th1 clone 103 and Th2 clone 2/46 were cultured in medium alone, or in the presence of immobilized anti-TCR MoAb, or treated with gp120/anti-gp120 and then cultured with immobilized anti-TCR (simply named gp120 in the figure), or stimulated with PMA/ionophore. After 1 hour, cells were collected, washed, and used as effectors in a 20-hour cytotoxicity test toward 51Cr labeled Jurkat cells, with or without the anti-CD95 neutralizing MoAb ZB4 (50 ng/mL). Effector to target ratio was 20:1. Mean ± SD of three replicates is shown.
