Fig. 4.
Changes in p15 and BPI mRNA levels in blood and exudate cells after induction of inflammatory exudate. (A) Northern blots of p15 and BPI mRNA isolated from equivalent numbers of inflammatory exudate cells collected at indicated times after induction of the inflammatory response (see Materials and Methods). (B) RT-PCR analysis of mRNAs isolated from equivalent numbers of peripheral blood (B) or inflammatory exudate (X) cells as described in Materials and Methods and the legend to Fig 2. T-0 shows baseline mRNA levels in a blood sample collected immediately before injection of the inflammatory stimulus. Thereafter, blood and exudate samples were collected in parallel at the times indicated (in hours). Results shown are representative of two similar experiments.