Anti–IFN-α neutralizing antibody blocks HCMV-induced suppression of monocyte oxidative activity. Anti–IFN-α or anti–IFN-β neutralizing polyclonal antibodies (100 U/mL) and inactivated cell-free HCMV (Toledo virus, 0.002 MOI equivalent) (dark bars) or the mock preparation (light bars) were added simultaneously to PBMCs cultures at day 0. Assays were performed in triplicate and the oxidative assay was performed at day 3. Three independent experiments were performed and results are presented as the mean ± SD of the OD values.