Multiplex PCR analysis of the RHD gene. This figure shows the products of the multiplex reaction after separation on 6% acrylamide gels. Products of 531 bp (RHCE intron 4); 299 bp (RHD intron 4) and 196 bp (RHD exon 10). Products are indicated between the two panels. (A) Illustrates samples that are concordant (all normal D⁺ and dce phenotypes; representing the majority of all samples tested) and (B) illustrates discordant samples. The Rh phenotype of each sample is indicated above each track. All samples on (A) and those on (B) were run on the same gel.