Expression of ADF in ATL cell lines. Cell lysates (40 μg) were electrophoresed by 12% SDS-PAGE under reducing conditions and transferred to polyvinylidenedifluoride membranes. Immunostaining was performed using anti-ADF antibody as the first antibody. (A) ATL-2, ATL-2/ADM cl-1, and ATL-2/ADM cl-2; (B) SALT-3, SALT-3/ADM cl-1, and SALT-3/ADM cl-2