Two-color analysis of immunofluorescent staining of T cells in the unfractionated cells from an additional donor in the presence or absence of unconjugated anti-CD32 MoAbs. In (A), cells were stained with PE-conjugated anti-CD4 and anti-CD8 MoAbs, and counter-stained with FITC-conjugated anti-CD3 antibodies in the absence of anti-CD32 antibodies. In (B), cells were stained in the presence of a saturating concentration of anti-CD32 antibodies (100 μg/mL).