Light scatter analysis of unfractionated and density fractionated leukapheresis cells after gating on T-cell subsets. The two panels on the left show the boxes used to gate on CD4⁻CD8⁻ T cells (A) or CD4⁺/CD8⁺ T cells (B). The remaining panels show the light scatter analysis of gated CD4⁺/CD8⁺ T cells or CD4⁻CD8⁻ T cells in unfractionated or density fractionated cells from the same donor. Boxes in the panels labeled U and C1-C5 enclose the “mononuclear/lymphocyte” gate, and the percentages of cells enclosed in the boxes are shown.