Fig. 7.
![Fig. 7. In vitro transcription, translation, and transport of ALAS-E precursor into mitochondria. Wild type (lanes 2 and 5) and V190 mutant ALAS-E (lanes 3 and 6) precursors were synthesized in the presence of [35S]-labeled methionine. Lanes 1 and 4 are controls in which the RNA was omitted. The translation products were incubated with mouse liver mitochondria and then centrifuged to collect the mitochondria. Supernatant (lanes 1 to 3) and mitochondrial (lanes 4 to 6) fractions were analyzed by SDS-PAGE. Note that the sizes of the precursors are similar, but the processed mutant proteins are larger than the wild type.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/2/10.1182_blood.v90.2.822/4/bl_0035f7.jpeg?Expires=1735497319&Signature=Ga6t5DI5PM870KcOP~hDxGU3~qun~YM2tyczrSoSqX2CL4lExsIGZo0ThVFwa9sYbjTF4f8LRznkeHnKE23DwgosOEiLZoPQhJ-DlQ5~CH3DDjKbLheWdlHEvYWm13iPXgHZRQ1BHZnaATa3OtdbVqfC8K58vNKWm52cvBe3qrEDdPcnEzJS8F9Rv6c9auz-gAk0t6gW40Caiaxy0LVcUdQG8D~gQCV3~8DBEWKIWOaEQiXq~1pH9gEjf4QyfBSAjd18fXn3DEyhv-HsACDTKj1540rG29BoQ4ix0h2IInHiOqnAyg2V4MKM6a-fnBc4baNHaGTlvviDm6YKvZjyPQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
In vitro transcription, translation, and transport of ALAS-E precursor into mitochondria. Wild type (lanes 2 and 5) and V190 mutant ALAS-E (lanes 3 and 6) precursors were synthesized in the presence of [35S]-labeled methionine. Lanes 1 and 4 are controls in which the RNA was omitted. The translation products were incubated with mouse liver mitochondria and then centrifuged to collect the mitochondria. Supernatant (lanes 1 to 3) and mitochondrial (lanes 4 to 6) fractions were analyzed by SDS-PAGE. Note that the sizes of the precursors are similar, but the processed mutant proteins are larger than the wild type.
