Expression of mRNAs for several Bcl-2–related genes in FVA cells. Each panel shows the steady-state levels of mRNA for the indicated gene. All panels represent the same blot probed successively with various probes. Each lane contained 20 μg of total RNA taken from FVA cells cultured with or without EP and harvested at the designated time points. The first panel shows the blot probed with a bcl-x probe. The second panel shows the blot reprobed without stripping with a bax probe (* band is residual bcl-x probe activity). The third panel shows the blot after stripping and reprobing with an A1 probe. The probes for bcl-x,bax, and A1 were approximately equal in size (∼600 bp) and all labeling and hybridization conditions were similar. Film exposure times were 24 hours for bcl-x and bax and 172 hours for A1. The fourth panel shows the blot stripped and reprobed with a 28S rRNA probe. The bottom panel shows the blot stripped and reprobed with a β-globin probe (designated Hb).