Fig. 3.
![Fig. 3. Scatchard plots of [125I]–GM-CSF binding to target cells. In (A), N87 cells (1.3 × 106/200 μL/well) were incubated with 0.15, 0.3, 0.6, 1.2, 2.4, and 4.8 nmol/L [125I]–GM-CSF (5.7 μCi/μg) in RPMI containing 0.1% bovine serum albumin and 0.2% NaN3 for 30 minutes, washed, and counted. HL60 cells (B) were assayed similarly at 2.2 × 106/well.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.252/5/bl_0029f3.jpeg?Expires=1735548056&Signature=H9tIEu~5qKGOYa8A32SObvVWQEd58T5UfrRk6tvc08s-6il78HxPoA0Z72qQwbhMYNiNlh~DgNZoKMATlASJH1FRs4fXl8-qZjozWlt~ZFuUS5VZyx129fSyrAnhUXPrMzZWe8cWFA0pyJQHZ5~sbcVkvgjvqjQ19FCixGWX66gG6v57NUx8Idk-etQu8ps2c2yhjthFpDODQ-3TfB2~yX5JkAZbpbyJlt6OcH8m-xAndLdQJhvL-ECDZIR88ULL05etkywVir--gnOQYfcq5qxD9Z6XSbsXpdK3W9GzxJI~qd3211LGgEqjM2Tjfdtvm8xW-jKcYG9rnNC6CwB7Fg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Scatchard plots of [125I]–GM-CSF binding to target cells. In (A), N87 cells (1.3 × 106/200 μL/well) were incubated with 0.15, 0.3, 0.6, 1.2, 2.4, and 4.8 nmol/L [125I]–GM-CSF (5.7 μCi/μg) in RPMI containing 0.1% bovine serum albumin and 0.2% NaN3 for 30 minutes, washed, and counted. HL60 cells (B) were assayed similarly at 2.2 × 106/well.
