Fig. 5.
![Fig. 5. Displacement analysis of recombinant toxins. In (A), U937 cells (4.5 × 106/well) were incubated with [125I]–GM-CSF and the indicated concentrations of GM-CSF (▪), GM-CSF–PE38KDEL (○), or DT388–GM-CSF (▵). In (B), HUT-102 cells (8 × 105/well) were incubated with [125I] — anti-TFR–IgG and the indicated concentrations of anti-TFR–IgG (▪), anti-TFR(Fv)–PE38KDEL (○), or DT388–anti-TFR(Fv) (▴). The cells were harvested and counted as in Fig 3.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.252/5/bl_0029f5.jpeg?Expires=1735547652&Signature=4vQlAOVX8N87b~i0Wi9QB5sL8FqSaKV9jd41OorKQhg6ABz6u6eWntwlHU-qUbpp6jnI4g6sYUES0j~OD2LXF0Hhivla6GNQXUESWwmTsc608xdj~nOxwrMSQlphSlQFUwB8WUsi7uEp5mwM4wpefPbFK7PzfPVZfQU5OlPqlJZvwFi7Jjl4tk9M8FTDJMjRqiWHBSzUSmMxrl5DRFtpIHq0-ObS5sgk-m2dSFHdyVwJrPNNJMyZ9opFYrLbaUoHmRwk7pjmN~jFoMnuWfw97YyquGruFrKfK-b~VbSnvzkupQGkMVJwSp11KPylf1l081imAyGE8LGeg~7nJrbmkA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Displacement analysis of recombinant toxins. In (A), U937 cells (4.5 × 106/well) were incubated with [125I]–GM-CSF and the indicated concentrations of GM-CSF (▪), GM-CSF–PE38KDEL (○), or DT388–GM-CSF (▵). In (B), HUT-102 cells (8 × 105/well) were incubated with [125I] — anti-TFR–IgG and the indicated concentrations of anti-TFR–IgG (▪), anti-TFR(Fv)–PE38KDEL (○), or DT388–anti-TFR(Fv) (▴). The cells were harvested and counted as in Fig 3.
