Fig. 6.
BCR-dependent B-cell activation antagonizes HLA class I-mediated apoptosis. Naive B cells were cultured on irradiated CD32-L cells in the presence of 1 μg/mL anti-CD40 MoAb alone, 5 μg/mL anti-BCR MoAb alone, or the combination of both. After 5 days of culture, cells were collected, treated by 5 μg/mL control IgG1, W6/32, MoAb90, YTH862, or 1 μg/mL anti-Fas MoAb CH11 for 2 hours and then the percentage of DNA fragmented cells was measured by TUNEL assay. Each histogram represents 104 cells and percentage of apoptotic cells are indicated above to the histogram profiles.
