Fig. 3.
![Fig. 3. Recombinant Sp1 protein bound the core promoter to form the same DNA-protein complexes as nuclear extracts from K562 cells or from K562 cells induced with phorbol dibutyrate. Gel mobility shift experiments were performed using 32P-end–labeled core promoter (bp −30 to −92) and either recombinant Sp1 protein (at the indicated footprinting units [fu]) or nuclear extracts from uninduced and induced K562 cells. A footprinting unit is defined as the amount of protein required to yield a complete footprint on a fragment of the SV40 early promoter. Two DNA-protein complexes are indicated by arrows.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/2/10.1182_blood.v90.2.678/4/bl_0025f3.jpeg?Expires=1735537657&Signature=f07yt78oUud-Db-9Bkaw5cWx8fIIudNrGHwPeImQSUrRxaXbLxyOuwdSJq7ZgEtlUEfCYITa0a~G5c4GOjLFoaI3lW7e3qbFiWkamK2lnIhMHTPgQ-CgYbTsVp2mzcIgWo2ilaE1~Xss4cFV3UkDAYdkJUKGSebjU10XkoKCWZJsVBP-7m9jh4bFoaqya0oAvDWB~mQ8nXe5jRG7tHKxZpViWFDmiN-D6cHDtNVSt3x5KPh2HQw0VbcHL8KV3fsKn1qbyddfa4Mwzf1w-nYkGJN9ulzoI30gTKqawLNINsmFwQSr70o0tmbGKUGGnjv2obfiNs~ceJhtkMjULoguPA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Recombinant Sp1 protein bound the core promoter to form the same DNA-protein complexes as nuclear extracts from K562 cells or from K562 cells induced with phorbol dibutyrate. Gel mobility shift experiments were performed using 32P-end–labeled core promoter (bp −30 to −92) and either recombinant Sp1 protein (at the indicated footprinting units [fu]) or nuclear extracts from uninduced and induced K562 cells. A footprinting unit is defined as the amount of protein required to yield a complete footprint on a fragment of the SV40 early promoter. Two DNA-protein complexes are indicated by arrows.
