American Society of Hematology

Fig. 4.

$Fig. 4. Detection of HPB-ALL DNA in brain and cervical lymph node. One microgram of DNA from either the brain or cervical lymph node of the same animal was subjected to amplification by PCR using the fluorescent primers described. One microliter of the resulting reaction mixture was subjected to fractionation on a 6% denaturant polyacrylamide gel containing 8 mol/L urea and run for 3 hours in the Model 373 Genetic Analyzer (Applied Biosystems) and the results analyzed by Genescanner software (Applied Biosystems) as described. Signals from the brain (open graph) and lymph node (darkened graph, arrow) have been superimposed to be on the same scale. The expected fragment size of the HPB-ALL T-cell receptor VDJ region being amplified is 112 bases. After correcting for the recovery of standards, the relative areas of the brain and lymph node peaks are in the approximate ratio of 100:1.$

Detection of HPB-ALL DNA in brain and cervical lymph node. One microgram of DNA from either the brain or cervical lymph node of the same animal was subjected to amplification by PCR using the fluorescent primers described. One microliter of the resulting reaction mixture was subjected to fractionation on a 6% denaturant polyacrylamide gel containing 8 mol/L urea and run for 3 hours in the Model 373 Genetic Analyzer (Applied Biosystems) and the results analyzed by Genescanner software (Applied Biosystems) as described. Signals from the brain (open graph) and lymph node (darkened graph, arrow) have been superimposed to be on the same scale. The expected fragment size of the HPB-ALL T-cell receptor VDJ region being amplified is 112 bases. After correcting for the recovery of standards, the relative areas of the brain and lymph node peaks are in the approximate ratio of 100:1.

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