Flow cytometric analysis of surface marker expression on Lin⁻CD34⁺-enriched CB cells before (A) and after (B) 1 week of incubation on pre-established allogeneic stroma. (A1 through A3) Freshly isolated Lin⁻CD34⁺-enriched CB cells. (A1) Forward and side scatter plot showing the gate (R1) used for the analysis. (A2) Isotype control for nonspecific IgG1 staining. (A3) CD34 and CD38 expression on the cells gated in R1. (B1 through B3) Lin⁻CD34⁺-enriched CB cells were cultured for 1 week. (B1) Forward and side scatter plot showing the gate (R1) used for the analysis. (B2) Isotype control for nonspecific IgG1 staining. (B3) CD34 and CD38 expression on the cells gated in R1.