Fig. 3.
HPLC profile of metabolites recovered from the urine of a normal subject taking DCF (———) and of DCF and 4′-OH DCF standards assayed separately (– – – –). The five bracketed fractions were tested for the ability to promote agglutination of normal RBCs in the presence of serum from our patient. As shown above each bracket, only the fraction eluting at ∼15.1 minutes caused RBC agglutination (4+). Negative results were obtained when material in each peak was tested with normal plasma (not shown).