Fig. 5.
![Fig. 5. CrmA expression protects CEM-C7H2 T cells from being killed by malignant plasma cells. CrmA-transfected T cells (clone 2E8, □) and T cells carrying the relevant vector control (clone IC3, ▪) were labeled with 3[H]-thymidine for 5 hours and cocultivated with the unlabeled neoplastic plasma cell lines for 72 hours using the predetermined E/T ratio of 10:1. Treatment of T cells with 0.25 μg/mL anti-Fas MoAb served as an internal control for the assay. Mean values ± SEM (n = 8) are given. The decrease in DNA-incorporated radioactivity was used to calculate the percentage of myeloma cell–mediated target cell death. Mean values ± SEM (n = 8) are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.12/5/bl_0055f5.jpeg?Expires=1736042622&Signature=d2B1j-IpSMlMH5P7R22HujbrcY61VpuTEA62yqXCy0kgmi3gvJIQA9oTuD~LaVDorOscaWR73YmKnMUBdE7CqxOn5Cj0lXEx5cpZxG-Npt-ATSuaiIhx5G0rQ434Z~-XYctpWwMHFfAnQnMbUjUwdu4Dehz3whvFXCvPB64K1b9uT9bIyNd-kZU~5JdmC4lYA9TDvyqtJPjvHJqKJNY9B9-lJhclM8FGUxP4mH69XdiMrHvfj3H-kDQAwEnfOIiiUjzoQ3Xsd9-EwV7D17C2Y8X2VRvc3lOJoiMDWuG50ydOmK2TajmG3XZDvUDDE-Mx7aS3G0kUUwz6R6B9A-MKLg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CrmA expression protects CEM-C7H2 T cells from being killed by malignant plasma cells. CrmA-transfected T cells (clone 2E8, □) and T cells carrying the relevant vector control (clone IC3, ▪) were labeled with 3[H]-thymidine for 5 hours and cocultivated with the unlabeled neoplastic plasma cell lines for 72 hours using the predetermined E/T ratio of 10:1. Treatment of T cells with 0.25 μg/mL anti-Fas MoAb served as an internal control for the assay. Mean values ± SEM (n = 8) are given. The decrease in DNA-incorporated radioactivity was used to calculate the percentage of myeloma cell–mediated target cell death. Mean values ± SEM (n = 8) are shown.
