Fig. 4.
Fig. 4. PCR assay to detect IgH DJ rearrangements in sorted FBM B-lineage subpopulations. (A)FBM DHQ52 DJ rearrangements. (B) FBM DXP DJ rearrangements. No germline band is observed in the DXP rearrangements lane because the primers anneal to sequences that are widely separated (20 kb) in unrearranged DNA. Shown are results representative of repeated PCR analysis of three independently sorted FBM samples and two independently sorted ABM samples.

PCR assay to detect IgH DJ rearrangements in sorted FBM B-lineage subpopulations. (A)FBM DHQ52 DJ rearrangements. (B) FBM DXP DJ rearrangements. No germline band is observed in the DXP rearrangements lane because the primers anneal to sequences that are widely separated (20 kb) in unrearranged DNA. Shown are results representative of repeated PCR analysis of three independently sorted FBM samples and two independently sorted ABM samples.

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