Fig. 4.
![Fig. 4. Effects of UV irradiation on [3H]ara-C incorporation into DNA. Exponentially growing CCRF-CEM cells were treated without (○), or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.6 nmol/L [3H]ara-C (1.5 × 10−2 μCi/mL) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 4 hours (A) and 13 hours (B). DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/1/10.1182_blood.v90.1.270/5/bl_0051f4.jpeg?Expires=1735554312&Signature=D3VQRlDD~aAsW4MrQw587rhDVRvVcmZHPBk9GaAhilFOoPRWBQdk8Z0R1iO5qZ~baf4G2CX0~1cu1hrtCEGBxGxLEUZMtFNkF7r~FCdDUscj~AFThjOH-Mi04xyOt6eWWSQ77ZUY10CVMqWC2WZ1C2cmGc6SVr637ojyPwPiXKmtN43upl79lzBIg-~7M6OqKGT0IJ2xUCJxuNpUg46gr~qbizY~wCKGFr2D85WrVIQIVQD1MPpZIaNDlpX50L8ZebsdZN9rQUibkYW1MWbFEMBDKfrICzq-1ln6xLVsbdOQkjh079LIUnDwezCsqNOYkWq59Rv1C0dIp3U5Dr-mUw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of UV irradiation on [3H]ara-C incorporation into DNA. Exponentially growing CCRF-CEM cells were treated without (○), or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.6 nmol/L [3H]ara-C (1.5 × 10−2 μCi/mL) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 4 hours (A) and 13 hours (B). DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.
