Fig. 5.
Effect of UV irradiation on the incorporation of dFdC or F-ara-A. Exponentially growing CCRF-CEM cells were treated without (○) or with 10 J/m2 UV light (•). Then cells were immediately labeled with 0.8 nmol/L [3H]dFdC (1.5 × 10−2 μCi/mL) (A) and 120 nmol/L [3H]F-ara-A (1.5 μCi/mL) (B) in the presence of 1.0 μmol/L BrdUrd and 10 nmol/L FdUrd for 13 hours. DNA isolation and CsCl ultracentrifugation were performed as described in Materials and Methods. Fractions were collected for the measurement of radioactivity.