RT-PCR cytokine analysis of GzmB-Tax transgenic tumors and derived LGL cell lines. The first and third panels show mRNA expression of IL-1α, IL-1β, IFNγ, and GM-CSF in the peripheral tumors of two different transgenic mice (m388 Tumor and f197 Tumor). The second and fourth panels show that the tumor-derived LGL cell lines express mRNAs for only IFNγ and GM-CSF (m388 LGL Cell Line and f197 LGL Cell Line). Negative and positive RT-PCR controls are shown in each panel and are labeled as HPGRT(−) and HPGRT(+), respectively. Excess primer for each RT-PCR reaction is indicated at the right side of each panel (primer).