Influence of rCD44v4-v7 on the regain of responsiveness after syngeneic and allogeneic reconstitution. TC, SC, and LNC of lethally irradiated syngeneic C57BL/6 and allogeneic BALB/c mice that were reconstituted with BMC from NTG and rCD44v4-v7 TG C57BL/6 mice were collected 2 to 8 weeks after BMC transfer. Cells from 3 mice per group were pooled and were cultured in triplicates for 3 days in the presence of TNP-OA. Where indicated, lethally irradiated and reconstituted mice had received anti-rCD44v6 (200 μg twice per week). During the last 8 hours of culture, 3H-thymidine was added, cells were harvested, and incorporation of 3H-thymidine was determined in a β-counter. Background values were subtracted (cpm in the absence of an antigenic stimulus). Mean cpm ± SD of triplicate cultures are shown; significant differences between NTG and TG BMC (P ≤ .01) are indicated by an asterisk. The experiment was repeated three times showing similar results.
