Fig. 5.
Leukocyte distribution after reconstitution of allogeneic mice with BMC from rCD44v4-v7-TG and NTG mice. Lethally irradiated allogeneic BALB/c mice were reconstituted with 5 × 106 BMC from NTG and rCD44v4-v7 TG C57BL/6 mice. Mice received, in addition, either a control IgG1 or anti-rCD44v6 (200 μg twice per week). TC, SC, and LNC were harvested after 2 to 6 weeks. Expression of CD4, CD8, sIgM, H-2d (host), H-2b (donor), and the transgene product was analyzed by flow cytometry. The mean percentage of stained cells in 2 pools (TC and LNC from 3 mice, 2 weeks after reconstitution) or in 6 individually tested mice are shown. SD were in the range of 3% to 7%. Significant differences between NTG and TG BMC (P ≤ .01) are indicated by an asterisk.